Description：Rabbit polyclonal antibody to NEFHImmunogen：Recombinant full length protein of human NEFHPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/50)Gene Symbol：NEFHAlternative Names：KIAA0845; NFH; Neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; Neurofilament triplet H protein Entrez Gene (Human)： 4744; Entrez Gene (Mouse)： 380684; SwissProt (Human)： P12036; SwissProt (Mouse)： P19246; SwissProt (Rat)： P16884; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NEFH expression in mouse brain (A) whole cell lysates. (Predicted band size: 105; 112 kD; Observed band size: 150; 240 kD)
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  Immunofluorescent analysis of NEFH staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).