Description:Rabbit polyclonal antibody to MARK1Immunogen:Recombinant full length protein of human MARK1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol:MARK1Alternative Names:KIAA1477; MARK; Serine/threonine-protein kinase MARK1; MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c; Par-1c; Par1c
Entrez Gene (Human):
4139;
Entrez Gene (Mouse):
226778;
SwissProt (Human):
Q9P0L2;
SwissProt (Mouse):
Q8VHJ5;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MARK1 expression in Hela (A) whole cell lysates. (Predicted band size: 72; 84; 89 kD; Observed band size: 90; 72 kD)
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Immunohistochemical analysis of MARK1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.