Description:Rabbit polyclonal antibody to Vasopressin V1a ReceptorImmunogen:Recombinant full length protein of human Vasopressin V1a ReceptorPurification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:AVPR1AAlternative Names:AVPR1; Vasopressin V1a receptor; V1aR; AVPR V1a; Antidiuretic hormone receptor 1a; Vascular/hepatic-type arginine vasopressin receptor
Entrez Gene (Human):
552;
Entrez Gene (Mouse):
54140;
Entrez Gene (Rat):
25107;
SwissProt (Human):
P37288;
SwissProt (Mouse):
Q62463;
SwissProt (Rat):
P30560;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Vasopressin V1a Receptor expression in A549 (A), mouse liver (B), rat liver (C) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 60 kD)
-
Immunofluorescent analysis of Vasopressin V1a Receptor staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).