Description：Rabbit polyclonal antibody to CD163Immunogen：Recombinant full length protein of human CD163Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：CD163Alternative Names：M130; Scavenger receptor cysteine-rich type 1 protein M130; Hemoglobin scavenger receptor; CD163 Entrez Gene (Human)： 9332; Entrez Gene (Mouse)： 93671; SwissProt (Human)： Q86VB7; SwissProt (Mouse)： Q2VLH6; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD163 expression in SW480 (A), mouse lung (B), rat liver (C) whole cell lysates. (Predicted band size: 121; 124; 125 kD; Observed band size: 150 kD)
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  Immunohistochemical analysis of CD163 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of CD163 staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).