Description:Rabbit polyclonal antibody to RNF166Immunogen:KLH-conjugated synthetic peptide of human RNF166Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol:RNF166Alternative Names:RING finger protein 166
Entrez Gene (Human):
115992;
Entrez Gene (Mouse):
68718;
SwissProt (Human):
Q96A37;
SwissProt (Mouse):
Q3U9F6;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RNF166 expression in SHSY5Y (A), K562 (B), MCF7 (C) whole cell lysates. (Predicted band size: 14; 17; 26 kD; Observed band size: 26 kD)
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Immunohistochemical analysis of RNF166 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.