Description：Rabbit polyclonal antibody to NEDD4LImmunogen：Recombinant full length protein of human NEDD4LPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：NEDD4LAlternative Names：KIAA0439; NEDL3; E3 ubiquitin-protein ligase NEDD4-like; NEDD4.2; Nedd4-2 Entrez Gene (Human)： 23327; SwissProt (Human)： Q96PU5; SwissProt (Mouse)： Q8CFI0; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NEDD4L expression in BT474 (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 96-111 kD; Observed band size: 113 kD)
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  Immunohistochemical analysis of NEDD4L staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of NEDD4L staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).