Description：Rabbit polyclonal antibody to HELIC1Immunogen：Recombinant full length protein of human HELIC1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：ASCC3Alternative Names：HELIC1; Activating signal cointegrator 1 complex subunit 3; ASC-1 complex subunit p200; ASC1p200; Helicase, ATP binding 1; Trip4 complex subunit p200 Entrez Gene (Human)： 10973; Entrez Gene (Mouse)： 77987; SwissProt (Human)： Q8N3C0; SwissProt (Mouse)： E9PZJ8; SwissProt (Rat)： F1LPQ2; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HELIC1 expression in Jurkat (A), Hela (B), mouse liver (C), mouse brain (D) whole cell lysates. (Predicted band size: 13; 83; 251 kD; Observed band size: 155kD)
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  Immunofluorescent analysis of HELIC1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.