Description:Rabbit polyclonal antibody to UBE2AImmunogen:Recombinant full length protein of human UBE2APurification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol:UBE2AAlternative Names:RAD6A; Ubiquitin-conjugating enzyme E2 A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A
Entrez Gene (Human):
7319;
Entrez Gene (Mouse):
22209;
SwissProt (Human):
P49459;
SwissProt (Mouse):
Q9Z255;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of UBE2A expression in mouse kidney (A) whole cell lysates. (Predicted band size: 8; 13; 17 kD; Observed band size: 14 kD)
-
Immunohistochemical analysis of UBE2A staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.