Description：Rabbit polyclonal antibody to Myosin 7Immunogen：Recombinant full length protein of human Myosin 7Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：MYH7Alternative Names：MYHCB; Myosin-7; Myosin heavy chain 7; Myosin heavy chain slow isoform; MyHC-slow; Myosin heavy chain, cardiac muscle beta isoform; MyHC-beta Entrez Gene (Human)： 4625; Entrez Gene (Mouse)： 140781; Entrez Gene (Rat)： 29557; SwissProt (Human)： P12883; SwissProt (Mouse)： Q91Z83; SwissProt (Rat)： P02564; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Myosin 7 expression in mouse skeletal muscle (A), rat skeletal muscle (B) whole cell lysates. (Predicted band size: 223 kD; Observed band size: 250 kD)
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  Immunofluorescent analysis of Myosin 7 staining in rat skeletal muscle. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).