Description:Rabbit polyclonal antibody to IFNGR2Immunogen:Recombinant full length protein of human IFNGR2Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:IFNGR2Alternative Names:IFNGT1; Interferon gamma receptor 2; IFN-gamma receptor 2; IFN-gamma-R2; Interferon gamma receptor accessory factor 1; AF-1; Interferon gamma transducer 1
Entrez Gene (Human):
3460;
SwissProt (Human):
P38484;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IFNGR2 expression in mouse brain (A) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 38 kD)
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Immunofluorescent analysis of IFNGR2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).