Description：Rabbit polyclonal antibody to ATG14Immunogen：Recombinant full length protein of human ATG14Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：ATG14Alternative Names：KIAA0831; Beclin 1-associated autophagy-related key regulator; Barkor; Autophagy-related protein 14-like protein; Atg14L Entrez Gene (Human)： 22863; Entrez Gene (Mouse)： 100504663; Entrez Gene (Rat)： 305831; SwissProt (Human)： Q6ZNE5; SwissProt (Mouse)： Q8CDJ3; SwissProt (Rat)： D4A4K3; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ATG14 expression in Hela (A), mouse liver (B), rat brain (C), rat lung (D) whole cell lysates. (Predicted band size: 42; 55 kD; Observed band size: 60 kD)
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  Immunofluorescent analysis of ATG14 staining in BALB3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).