Description：Rabbit polyclonal antibody to BRKImmunogen：Recombinant full length protein of human BRKPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：PTK6Alternative Names：BRK; Protein-tyrosine kinase 6; Breast tumor kinase; Tyrosine-protein kinase BRK Entrez Gene (Human)： 5753; Entrez Gene (Mouse)： 20459; SwissProt (Human)： Q13882; SwissProt (Mouse)： Q64434; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of BRK expression in MCF7 (A), A549 (B), NIH3T3 (C), mouse liver (D), rat kidney (E) whole cell lysates. (Predicted band size: 14; 51 kD; Observed band size: 52 kD)
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  Immunofluorescent analysis of BRK staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.