Description:Rabbit polyclonal antibody to SULT1E1Immunogen:Recombinant full length protein of human SULT1E1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:SULT1E1Alternative Names:STE; Estrogen sulfotransferase; EST-1; Sulfotransferase 1E1; ST1E1; Sulfotransferase, estrogen-preferring
Entrez Gene (Human):
6783;
SwissProt (Human):
P49888;
SwissProt (Mouse):
P49891;
SwissProt (Rat):
P52844;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SULT1E1 expression in mouse liver (A), rat liver (B) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 35 kD)
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Immunofluorescent analysis of SULT1E1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.