Description：Rabbit polyclonal antibody to AMPK alpha 2Immunogen：Recombinant full length protein of human AMPK alpha 2Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：PRKAA2Alternative Names：AMPK; AMPK2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase Entrez Gene (Human)： 5563; Entrez Gene (Mouse)： 108079; Entrez Gene (Rat)： 78975; SwissProt (Human)： P54646; SwissProt (Mouse)： Q8BRK8; SwissProt (Rat)： Q09137; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of AMPK alpha 2 expression in SW620 (A), mouse brain (B), rat heart (C) whole cell lysates. (Predicted band size: 62 kD; Observed band size: 62 kD)
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  Immunofluorescent analysis of AMPK alpha 2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.