Description：Rabbit polyclonal antibody to TMLHEImmunogen：Recombinant full length protein of human TMLHEPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：TMLHEAlternative Names：TMLH; Trimethyllysine dioxygenase mitochondrial; Epsilon-trimethyllysine 2-oxoglutarate dioxygenase; Epsilon-trimethyllysine hydroxylase; TML hydroxylase; TML-alpha-ketoglutarate dioxygenase; TML dioxygenase; TMLD Entrez Gene (Human)： 55217; Entrez Gene (Mouse)： 192289; Entrez Gene (Rat)： 170898; SwissProt (Human)： Q9NVH6; SwissProt (Mouse)： Q91ZE0; SwissProt (Rat)： Q91ZW6; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TMLHE expression in HT29 (A), SKOV3 (B), mouse heart (C), rat liver (D) whole cell lysates. (Predicted band size: 38; 41; 42; 44; 46; 49; 50 kD; Observed band size: 50 kD)
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  Immunofluorescent analysis of TMLHE staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.