Description：Rabbit polyclonal antibody to Histone H3 (TriMethyl-K56)Immunogen：KLH-conjugated synthetic Tri-methylated peptide corresponding to residues surrounding K56 of human Histone H3 protein. The exact sequence is proprietary.Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200), IP (1/20 - 1/50)Gene Symbol：HIST3H3Alternative Names：H3FT; Histone H3.1t; H3/t; H3t; H3/g Entrez Gene (Human)： 8290; SwissProt (Human)： Q16695; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Histone H3 (TriMethyl-K56) expression in HEK293T (A) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 16 kD)
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  Immunofluorescent analysis of Histone H3 (TriMethyl-K56) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).