Description:Rabbit polyclonal antibody to PINK1Immunogen:Recombinant full length protein of human PINK1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:PINK1Alternative Names:Serine/threonine-protein kinase PINK1 mitochondrial; BRPK; PTEN-induced putative kinase protein 1
Entrez Gene (Human):
65018;
Entrez Gene (Mouse):
68943;
SwissProt (Human):
Q9BXM7;
SwissProt (Mouse):
Q99MQ3;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PINK1 expression in U87 (A), mouse brain (B), mouse heart (C) whole cell lysates. (Predicted band size: 30; 62 kD; Observed band size: 63 kD)
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Immunofluorescent analysis of PINK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.