Description:Rabbit polyclonal antibody to ALS2CR6Immunogen:Recombinant full length protein of human ALS2CR6Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:ALS2Alternative Names:ALS2CR6; KIAA1563; Alsin; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 6 protein; Amyotrophic lateral sclerosis 2 protein
Entrez Gene (Human):
57679;
Entrez Gene (Mouse):
74018;
SwissProt (Human):
Q96Q42;
SwissProt (Mouse):
Q920R0;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ALS2CR6 expression in HL60 (A), SKOV3 (B), mouse brain (C) whole cell lysates. (Predicted band size: 42; 86; 183 kD; Observed band size: 184 kD)
-
Immunofluorescent analysis of ALS2CR6 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.