Description：Rabbit polyclonal antibody to EAF2Immunogen：Recombinant full length protein of human EAF2Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：EAF2Alternative Names：TRAITS; ELL-associated factor 2; Testosterone-regulated apoptosis inducer and tumor suppressor protein Entrez Gene (Human)： 55840; Entrez Gene (Mouse)： 106389; Entrez Gene (Rat)： 266787; SwissProt (Human)： Q96CJ1; SwissProt (Mouse)： Q91ZD6; SwissProt (Rat)： Q811X5; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EAF2 expression in HepG2 (A), A431 (B), mouse kidney (C), rat kidney (D) whole cell lysates. (Predicted band size: 14; 28 kD; Observed band size: 28 kD)
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  Immunofluorescent analysis of EAF2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.