Description：Rabbit polyclonal antibody to CD275Immunogen：Recombinant full length protein of human CD275Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol：ICOSLGAlternative Names：B7H2; B7RP1; ICOSL; KIAA0653; ICOS ligand; B7 homolog 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD275 Entrez Gene (Human)： 23308; Entrez Gene (Mouse)： 50723; SwissProt (Human)： O75144; SwissProt (Mouse)： Q9JHJ8; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD275 expression in HL60 (A), mouse brain (B), rat spleen (C) whole cell lysates. (Predicted band size: 20; 33; 34 kD; Observed band size: 60 kD)
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  Immunohistochemical analysis of CD275 staining in human skin cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.