Description：Rabbit polyclonal antibody to TRAF4Immunogen：Recombinant full length protein of human TRAF4Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)Gene Symbol：TRAF4Alternative Names：CART1; MLN62; RNF83; TNF receptor-associated factor 4; Cysteine-rich domain associated with RING and Traf domains protein 1; Metastatic lymph node gene 62 protein; MLN 62; RING finger protein 83 Entrez Gene (Human)： 9618; Entrez Gene (Mouse)： 22032; SwissProt (Human)： Q9BUZ4; SwissProt (Mouse)： Q61382; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TRAF4 expression in HepG2 (A) whole cell lysates. (Predicted band size: 22; 53 kD; Observed band size: 54 kD)
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  Immunofluorescent analysis of TRAF4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.