Description：Rabbit polyclonal antibody to USP8Immunogen：Recombinant full length protein of human USP8Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：USP8Alternative Names：KIAA0055; UBPY; Ubiquitin carboxyl-terminal hydrolase 8; Deubiquitinating enzyme 8; Ubiquitin isopeptidase Y; hUBPy; Ubiquitin thioesterase 8; Ubiquitin-specific-processing protease 8 Entrez Gene (Human)： 9101; Entrez Gene (Mouse)： 84092; SwissProt (Human)： P40818; SwissProt (Mouse)： Q80U87; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of USP8 expression in MCF7 (A), HT29 (B), mouse testis (C) whole cell lysates. (Predicted band size: 115; 127 kD; Observed band size: 128 kD)
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  Immunofluorescent analysis of USP8 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.