Description:Rabbit polyclonal antibody to SMC1AImmunogen:Recombinant full length protein of human SMC1APurification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:SMC1AAlternative Names:DXS423E; KIAA0178; SB1.8; SMC1; SMC1L1; Structural maintenance of chromosomes protein 1A; SMC protein 1A; SMC-1-alpha; SMC-1A; Sb1.8
Entrez Gene (Human):
8243;
Entrez Gene (Mouse):
24061;
Entrez Gene (Rat):
63996;
SwissProt (Human):
Q14683;
SwissProt (Mouse):
Q9CU62;
SwissProt (Rat):
Q9Z1M9;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SMC1A expression in Hela (A), SKOV3 (B), mouse liver (C), rat liver (D) whole cell lysates. (Predicted band size: 143 kD; Observed band size: 143 kD)
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Immunofluorescent analysis of SMC1A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.