Description:Rabbit polyclonal antibody to CDC25Immunogen:Recombinant full length protein of human CDC25Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol:RASGRF1Alternative Names:RASGRF1; CDC25; GNRP; GRF1; Ras-specific guanine nucleotide-releasing factor 1; Ras-GRF1; Guanine nucleotide-releasing protein; GNRP; Ras-specific nucleotide exchange factor CDC25
Entrez Gene (Human):
5923;
Entrez Gene (Mouse):
19417;
SwissProt (Human):
Q13972;
SwissProt (Mouse):
P27671;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDC25 expression in mouse brain (A) whole cell lysates. (Predicted band size: 55; 143; 145 kD; Observed band size: 145 kD)
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Immunohistochemical analysis of CDC25 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.