Description：Rabbit polyclonal antibody to DGAT1Immunogen：KLH-conjugated synthetic peptide of human DGAT1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：DGAT1Alternative Names：AGRP1; DGAT; Diacylglycerol O-acyltransferase 1; ACAT-related gene product 1; Acyl-CoA retinol O-fatty-acyltransferase; ARAT; Retinol O-fatty-acyltransferase; Diglyceride acyltransferase Entrez Gene (Human)： 8694; Entrez Gene (Mouse)： 13350; Entrez Gene (Rat)： 84497; SwissProt (Human)： O75907; SwissProt (Mouse)： Q9Z2A7; SwissProt (Rat)： Q9ERM3; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DGAT1 expression in mouse heart (A) whole cell lysates. (Predicted band size: 55 kD; Observed band size: 55 kD)
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  Immunofluorescent analysis of DGAT1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).