Description：Rabbit polyclonal antibody to CD231Immunogen：Recombinant full length protein of human CD231Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：TSPAN7Alternative Names：A15; DXS1692E; MXS1; TM4SF2; Tetraspanin-7; Tspan-7; Cell surface glycoprotein A15; Membrane component chromosome X surface marker 1; T-cell acute lymphoblastic leukemia-associated antigen 1; TALLA-1; Transmembrane 4 superfamily member 2; CD231 Entrez Gene (Human)： 7102; SwissProt (Human)： P41732; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD231 expression in K562 (A), BV2 (B), mouse spleen (C) whole cell lysates. (Predicted band size: 27 kD; Observed band size: 43, 28 kD)
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  Immunofluorescent analysis of CD231 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.