Description:Rabbit polyclonal antibody to Peroxiredoxin 5Immunogen:Recombinant full length protein of human Peroxiredoxin 5Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:PRDX5Alternative Names:ACR1; Peroxiredoxin-5 mitochondrial; Alu corepressor 1; Antioxidant enzyme B166; AOEB166; Liver tissue 2D-page spot 71B; PLP; Peroxiredoxin V; Prx-V; Peroxisomal antioxidant enzyme; TPx type VI; Thioredoxin peroxidase PMP20; Thioredoxin reductase
Entrez Gene (Human):
25824;
Entrez Gene (Mouse):
54683;
Entrez Gene (Rat):
113898;
SwissProt (Human):
P30044;
SwissProt (Mouse):
P99029;
SwissProt (Rat):
Q9R063;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Peroxiredoxin 5 expression in Hela (A), HepG2 (B), mouse liver (C), mouse heart (D), rat brain (E) whole cell lysates. (Predicted band size: 12; 17; 22 kD; Observed band size: 17 kD)
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Immunofluorescent analysis of Peroxiredoxin 5 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.