Description:Rabbit polyclonal antibody to Kv1.2Immunogen:Recombinant full length protein of human Kv1.2Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:KCNA2Alternative Names:Potassium voltage-gated channel subfamily A member 2; NGK1; Voltage-gated K(+) channel HuKIV; Voltage-gated potassium channel HBK5; Voltage-gated potassium channel subunit Kv1.2
Entrez Gene (Human):
3737;
Entrez Gene (Mouse):
16490;
SwissProt (Human):
P16389;
SwissProt (Mouse):
P63141;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Kv1.2 expression in mouse brain (A) whole cell lysates. (Predicted band size: 40; 56 kD; Observed band size: 56 kD)
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Immunofluorescent analysis of Kv1.2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.