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CQA3152
  • Western blot analysis of PRPF4 expression in HT29 (A), MCF7 (B), mouse testis (C), rat spleen (D) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 58 kD)
  • Immunohistochemical analysis of PRPF4 staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-PRPF4 Antibody
Cat No:CQA3152
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
50 μl
150
30 μl
110
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Description:Rabbit polyclonal antibody to PRPF4
Immunogen:Recombinant full length protein of human PRPF4
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)
Gene Symbol:PRPF4
Alternative Names:PRP4; U4/U6 small nuclear ribonucleoprotein Prp4; PRP4 homolog; hPrp4; U4/U6 snRNP 60 kDa protein; WD splicing factor Prp4
Entrez Gene (Human): 9128;
Entrez Gene (Mouse): 70052;
SwissProt (Human): O43172;
SwissProt (Mouse): Q9DAW6;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of PRPF4 expression in HT29 (A), MCF7 (B), mouse testis (C), rat spleen (D) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 58 kD)
  • Immunohistochemical analysis of PRPF4 staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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