Description：Rabbit polyclonal antibody to eTF1Immunogen：Recombinant full length protein of human eTF1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：ETF1Alternative Names：ERF1; RF1; SUP45L1; Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; eRF1; Protein Cl1; TB3-1 Entrez Gene (Human)： 2107; Entrez Gene (Mouse)： 225363; Entrez Gene (Rat)： 307503; SwissProt (Human)： P62495; SwissProt (Mouse)： Q8BWY3; SwissProt (Rat)： Q5U2Q7; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of eTF1 expression in SKOV3 (A), mouse liver (B), rat heart (C) whole cell lysates. (Predicted band size: 45; 49 kD; Observed band size: 53 kD)
• 
  Immunofluorescent analysis of eTF1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).