Description：Rabbit polyclonal antibody to RhoT1Immunogen：Recombinant full length protein of human RhoT1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：RHOT1Alternative Names：ARHT1; Mitochondrial Rho GTPase 1; MIRO-1; hMiro-1; Rac-GTP-binding protein-like protein; Ras homolog gene family member T1 Entrez Gene (Human)： 55288; Entrez Gene (Mouse)： 59040; SwissProt (Human)： Q8IXI2; SwissProt (Mouse)： Q8BG51; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RhoT1 expression in A549 (A), mouse brain (B), mouse kidney (C) whole cell lysates. (Predicted band size: 28; 66; 70; 71; 74; 75; 79 kD; Observed band size: 80 kD)
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  Immunofluorescent analysis of RhoT1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).