Description：Rabbit polyclonal antibody to Gemin3Immunogen：Recombinant full length protein of human Gemin3Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：DDX20Alternative Names：DP103; GEMIN3; Probable ATP-dependent RNA helicase DDX20; Component of gems 3; DEAD box protein 20; DEAD box protein DP 103; Gemin-3 Entrez Gene (Human)： 11218; Entrez Gene (Mouse)： 53975; SwissProt (Human)： Q9UHI6; SwissProt (Mouse)： Q9JJY4; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of Gemin3 expression in MCF7 (A), HepG2 (B), mouse spleen (C), rat testis (D) whole cell lysates. (Predicted band size: 14; 92 kD; Observed band size: 110 kD)
• 
  Immunofluorescent analysis of Gemin3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.