Description：Rabbit polyclonal antibody to DNMT1Immunogen：Recombinant full length protein of human DNMT1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200), IP (1/50 - 1/100)Gene Symbol：DNMT1Alternative Names：AIM; CXXC9; DNMT; DNA (cytosine-5)-methyltransferase 1; Dnmt1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT Entrez Gene (Human)： 1786; Entrez Gene (Mouse)： 13433; SwissProt (Human)： P26358; SwissProt (Mouse)： P13864; SwissProt (Rat)： Q9Z330; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DNMT1 expression in SW480 (A) whole cell lysates. (Predicted band size: 144; 183; 184 kD; Observed band size: 200 kD)
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  Immunofluorescent analysis of DNMT1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.