Description：Rabbit polyclonal antibody to BACE1Immunogen：Recombinant full length protein of human BACE1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：BACE1Alternative Names：BACE; KIAA1149; Beta-secretase 1; Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2 Entrez Gene (Human)： 23621; Entrez Gene (Mouse)： 23821; Entrez Gene (Rat)： 29392; SwissProt (Human)： P56817; SwissProt (Mouse)： P56818; SwissProt (Rat)： P56819; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of BACE1 expression in MCF7 (A), THP1 (B), mouse brain (C), rat heart (D) whole cell lysates. (Predicted band size: 42; 45; 48; 51; 52; 55 kD; Observed band size: 62 kD)
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  Immunofluorescent analysis of BACE1 staining in U251MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.