Description：Rabbit polyclonal antibody to SETD8Immunogen：Recombinant full length protein of human SETD8Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：SETD8Alternative Names：KMT5A; PRSET7; SET07; SET8; N-lysine methyltransferase SETD8; H4-K20-HMTase SETD8; Histone-lysine N-methyltransferase SETD8; Lysine N-methyltransferase 5A; PR/SET domain-containing protein 07; PR-Set7; PR/SET07; SET domain-containing protein 8 Entrez Gene (Human)： 387893; Entrez Gene (Mouse)： 67956; SwissProt (Human)： Q9NQR1; SwissProt (Mouse)： Q2YDW7; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SETD8 expression in HEK293T (A) whole cell lysates. (Predicted band size: 39; 42 kD; Observed band size: 43 kD)
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  Immunofluorescent analysis of SETD8 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).