Description：Rabbit polyclonal antibody to ATPIF1Immunogen：Recombinant full length protein of human ATPIF1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：ATPIF1Alternative Names：ATPI; ATPase inhibitor mitochondrial; Inhibitor of F(1)F(o)-ATPase; IF(1); IF1 Entrez Gene (Human)： 93974; Entrez Gene (Mouse)： 11983; Entrez Gene (Rat)： 25392; SwissProt (Human)： Q9UII2; SwissProt (Mouse)： O35143; SwissProt (Rat)： Q03344; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ATPIF1 expression in MCF7 (A), Hela (B), mouse brain (C) whole cell lysates. (Predicted band size: 6; 7; 12 kD; Observed band size: 12 kD)
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  Immunofluorescent analysis of ATPIF1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).