Description:Rabbit polyclonal antibody to ASNA1Immunogen:Recombinant full length protein of human ASNA1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:ASNA1Alternative Names:ARSA; TRC40; ATPase ASNA1; Arsenical pump-driving ATPase; Arsenite-stimulated ATPase; Transmembrane domain recognition complex 40 kDa ATPase subunit; hARSA-I; hASNA-I
Entrez Gene (Human):
439;
Entrez Gene (Mouse):
56495;
SwissProt (Human):
O43681;
SwissProt (Mouse):
O54984;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ASNA1 expression in MCF7 (A), mouse kidney (B), mouse lung (C), rat brain (D) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 39 kD)
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Immunofluorescent analysis of ASNA1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).