Description:Rabbit polyclonal antibody to STAU2Immunogen:KLH-conjugated synthetic peptide of human STAU2Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:STAU2Alternative Names:Double-stranded RNA-binding protein Staufen homolog 2
Entrez Gene (Human):
27067;
Entrez Gene (Mouse):
29819;
Entrez Gene (Rat):
171500;
SwissProt (Human):
Q9NUL3;
SwissProt (Mouse):
Q8CJ67;
SwissProt (Rat):
Q68SB1;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of STAU2 expression in mouse heart (A), mouse liver (B) whole cell lysates. (Predicted band size: 11; 34; 43; 52; 55; 59; 62 kD; Observed band size: 52 kD)
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Immunofluorescent analysis of STAU2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).