Description：Rabbit polyclonal antibody to NGALImmunogen：KLH-conjugated synthetic peptide of human NGALPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：LCN2Alternative Names：HNL; NGAL; Neutrophil gelatinase-associated lipocalin; NGAL; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; Siderocalin LCN2; p25 Entrez Gene (Human)： 3934; Entrez Gene (Mouse)： 16819; Entrez Gene (Rat)： 170496; SwissProt (Human)： P80188; SwissProt (Mouse)： P11672; SwissProt (Rat)： P30152; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NGAL expression in SW480 (A), A549 (B) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 22 kD)
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  Immunofluorescent analysis of NGAL staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.