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CQA2773
  • Western blot analysis of BUB1 expression in HepG2 (A), MCF7 (B), Hela (C), mouse testis (D) whole cell lysates. (Predicted band size: 115; 120; 122 kD; Observed band size: 133 kD)
  • Immunohistochemical analysis of BUB1 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product Name:Anti-BUB1 Antibody
Cat No:CQA2773
Source:Rabbit
Reactivity:H, M, R
Applications:WB, IH
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Size
Price(USD)
200 μl
350
100 μl
220
50 μl
150
30 μl
110
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Description:Rabbit polyclonal antibody to BUB1
Immunogen:Recombinant full length protein of human BUB1
Purification:The antibody was purified by immunogen affinity chromatography.
Clonality:Polyclonal
Form:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)
Gene Symbol:BUB1
Alternative Names:BUB1L; Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A
Entrez Gene (Human): 699;
Entrez Gene (Mouse): 12235;
SwissProt (Human): O43683;
SwissProt (Mouse): O08901;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of BUB1 expression in HepG2 (A), MCF7 (B), Hela (C), mouse testis (D) whole cell lysates. (Predicted band size: 115; 120; 122 kD; Observed band size: 133 kD)
  • Immunohistochemical analysis of BUB1 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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