Description:Rabbit polyclonal antibody to CD162Immunogen:Recombinant full length protein of human CD162Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:SELPLGAlternative Names:P-selectin glycoprotein ligand 1; PSGL-1; Selectin P ligand; CD162
Entrez Gene (Human):
6404;
SwissProt (Human):
Q14242;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD162 expression in Jurkat (A) whole cell lysates. (Predicted band size: 43; 44 kD; Observed band size: 120 kD)
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Immunofluorescent analysis of CD162 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.