Description：Rabbit polyclonal antibody to UPF1Immunogen：KLH-conjugated synthetic peptide of human UPF1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/50)Gene Symbol：UPF1Alternative Names：KIAA0221; RENT1; Regulator of nonsense transcripts 1; ATP-dependent helicase RENT1; Nonsense mRNA reducing factor 1; NORF1; Up-frameshift suppressor 1 homolog; hUpf1 Entrez Gene (Human)： 5976; Entrez Gene (Mouse)： 19704; SwissProt (Human)： Q92900; SwissProt (Mouse)： Q9EPU0; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of UPF1 expression in SHSY5Y (A), K562 (B), mouse liver (C) whole cell lysates. (Predicted band size: 123; 124 kD; Observed band size: 130 kD)
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  Immunofluorescent analysis of UPF1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.