Datasheet
MSDSDescription：Rabbit polyclonal antibody to Apolipoprotein BImmunogen：Recombinant full length protein of human Apolipoprotein BPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol：APOBAlternative Names：Apolipoprotein B-100; Apo B-100
Entrez Gene (Human)：
338;
Entrez Gene (Mouse)：
238055;
Entrez Gene (Rat)：
54225;
SwissProt (Human)：
P04114;
SwissProt (Mouse)：
E9Q414;
SwissProt (Rat)：
Q7TMA5;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Apolipoprotein B expression in HepG2 (A), HL60 (B), mouse liver (C), rat kidney (D) whole cell lysates. (Predicted band size: 515 kD; Observed band size: 100 kD) -
Immunofluorescent analysis of Apolipoprotein B staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Arachidonic acid is associated with dyslipidemia and cholesterol-related lipoprotein metabolism signatures