Description:Rabbit polyclonal antibody to LAMTOR1Immunogen:Recombinant full length protein of human LAMTOR1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:LAMTOR1Alternative Names:C11orf59; PDRO; Ragulator complex protein LAMTOR1; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1; Lipid raft adaptor protein p18; Protein associated with DRMs and endosomes; p27Kip1-releasing factor from RhoA; p27RF-Rho
Entrez Gene (Human):
55004;
Entrez Gene (Mouse):
66508;
Entrez Gene (Rat):
100361543;
308869;
SwissProt (Human):
Q6IAA8;
SwissProt (Mouse):
Q9CQ22;
SwissProt (Rat):
Q6P791;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of LAMTOR1 expression in Hela (A), Jurkat (B), mouse brain (C) whole cell lysates. (Predicted band size: 17 kD; Observed band size: 18 kD)
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Immunofluorescent analysis of LAMTOR1 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).