Description:Rabbit polyclonal antibody to ATG5Immunogen:Recombinant full length protein of human ATG5Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IH (1/50 - 1/200)Gene Symbol:ATG5Alternative Names:APG5L; ASP; Autophagy protein 5; APG5-like; Apoptosis-specific protein
Entrez Gene (Human):
9474;
Entrez Gene (Mouse):
11793;
SwissProt (Human):
Q9H1Y0;
SwissProt (Mouse):
Q99J83;
SwissProt (Rat):
Q3MQ06;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ATG5 expression in HepG2 (A), NIH3T3 (B), mouse spleen (C), rat kidney (D) whole cell lysates. (Predicted band size: 22; 32 kD; Observed band size: 50 kD)
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Immunohistochemical analysis of ATG5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.