Datasheet
MSDSDescription：Rabbit polyclonal antibody to SIRT1Immunogen：Recombinant full length protein of human SIRT1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：SIRT1Alternative Names：SIR2L1; NAD-dependent protein deacetylase sirtuin-1; hSIRT1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2
Entrez Gene (Human)：
23411;
Entrez Gene (Mouse)：
93759;
SwissProt (Human)：
Q96EB6;
SwissProt (Mouse)：
Q923E4;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SIRT1 expression in Myla2059 (A), SGC7901 (B), mouse spleen (C), rat spleen (D) whole cell lysates. (Predicted band size: 81; 61 kD; Observed band size: 120; 100 kD) -
Immunofluorescent analysis of SIRT1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).