Description:Rabbit polyclonal antibody to SQSTM1Immunogen:Recombinant full length protein of human SQSTM1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol:SQSTM1Alternative Names:ORCA; OSIL; Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62
Entrez Gene (Human):
8878;
Entrez Gene (Mouse):
18412;
Entrez Gene (Rat):
113894;
SwissProt (Human):
Q13501;
SwissProt (Mouse):
Q64337;
SwissProt (Rat):
O08623;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SQSTM1 expression in Hela (A), Jurkat (B), mouse heart (C), rat liver (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 62 kD)
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Immunofluorescent analysis of p62 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).