Description：Rabbit polyclonal antibody to SQSTM1Immunogen：Recombinant full length protein of human SQSTM1Purification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：SQSTM1Alternative Names：ORCA; OSIL; Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62 Entrez Gene (Human)： 8878; Entrez Gene (Mouse)： 18412; Entrez Gene (Rat)： 113894; SwissProt (Human)： Q13501; SwissProt (Mouse)： Q64337; SwissProt (Rat)： O08623; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SQSTM1 expression in Hela (A), Jurkat (B), mouse heart (C), rat liver (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 62 kD)
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  Immunofluorescent analysis of p62 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).