Description:Rabbit polyclonal antibody to BDNFImmunogen:Recombinant full length protein of human BDNFPurification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:BDNFAlternative Names:Brain-derived neurotrophic factor; BDNF; Abrineurin
Entrez Gene (Human):
627;
Entrez Gene (Mouse):
12064;
Entrez Gene (Rat):
24225;
SwissProt (Human):
P23560;
SwissProt (Mouse):
P21237;
SwissProt (Rat):
P23363;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BDNF expression in A549 (A), mouse kidney (B) whole cell lysates. (Predicted band size: 27; 28; 29; 31; 37 kD; Observed band size: 15; 35 kD)
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Immunofluorescent analysis of BDNF staining in U251MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).