Description:Rabbit polyclonal antibody to MYT1Immunogen:Recombinant full length protein of human MYT1Purification:The antibody was purified by immunogen affinity chromatography.Clonality:PolyclonalForm:Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution:WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)Gene Symbol:MYT1Alternative Names:KIAA0835; KIAA1050; MTF1; MYTI; PLPB1; Myelin transcription factor 1; MyT1; Myelin transcription factor I; MyTI; PLPB1; Proteolipid protein-binding protein
Entrez Gene (Human):
4661;
Entrez Gene (Mouse):
17932;
SwissProt (Human):
Q01538;
SwissProt (Mouse):
Q8CFC2;
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of MYT1 expression in A375 (A), mouse brain (B), rat liver (C) whole cell lysates. (Predicted band size: 223; 224; 227; 228 kD; Observed band size: 250 kD)
-
Immunofluorescent analysis of MYT1 staining in human placenta. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).