Description：Rabbit polyclonal antibody to SDHBImmunogen：Recombinant full length protein of human SDHBPurification：The antibody was purified by immunogen affinity chromatography.Clonality：PolyclonalForm：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)Gene Symbol：SDHBAlternative Names：SDH; SDH1; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit mitochondrial; Iron-sulfur subunit of complex II; Ip Entrez Gene (Human)： 6390; Entrez Gene (Mouse)： 67680; Entrez Gene (Rat)： 298596; SwissProt (Human)： P21912; SwissProt (Mouse)： Q9CQA3; SwissProt (Rat)： P21913; Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SDHB expression in HepG2 (A), Hela (B), mouse heart (C), rat liver (D), rat brain (E) whole cell lysates. (Predicted band size: 31 kD; Observed band size: 32 kD)
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  Immunofluorescent analysis of SDHB staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).